Thermo Fisher Scientific alpha Actinin 2 Polyclonal Antibody
Human, Mouse, Rat 반응성을 가진 Rabbit Polyclonal Antibody로 다양한 조직 및 세포에서 alpha Actinin 2 단백질 검출에 사용 가능. Western blot, IHC, ICC/IF에 적용 가능하며 고순도 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Thermo Fisher Scientific alpha Actinin 2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 215–530 of Human alpha Actinin 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546722 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, NIH-3T3, Rat heart
- Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (89%), Pig (99%), Chicken (97%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha actinins belong to the spectrin gene superfamily, a diverse group of cytoskeletal proteins including alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types.
- In nonmuscle cells: localized along microfilament bundles and adherens-type junctions, binding actin to the membrane.
- In muscle cells: skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, known to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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