Thermo Fisher Scientific ALF Monoclonal Antibody (3E4)
인간 ALF 단백질을 인식하는 Thermo Fisher의 3E4 클론 단일클론 항체입니다. Western blot, ICC/IF, ELISA에 사용 가능하며, 비결합형 액상 형태로 제공됩니다. PBS 버퍼에 보존제 없이 -20°C에서 보관하며, 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E4 |
| Immunogen | ALF (NP_006863, 251–348 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
PQVSQTNSNV ESVLSGSASM AQNLHDESLS TSPHGALHQH VTDIQLHILK NRMYGCDSVK QPRNIEEPSN IPVSEKDSNS QVDLSIRVTD DDIGEIIQ
Target Information
The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA.
This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that stabilizes TBP-DNA binding and may be uniquely important in testis biology.
Alternative splicing produces two distinct isoforms. Co-transcription with the neighboring upstream gene generates a rare transcript (SALF), encoding a fusion protein with sequence identity to both gene products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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