Thermo Fisher Scientific Notch2 Polyclonal Antibody
Human NOTCH2 단백질을 인식하는 Rabbit Polyclonal Antibody로 다양한 응용(WB, IHC, Flow, IP)에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. FFPE 조직에서는 citrate buffer pH 6.0을 이용한 epitope retrieval 권장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Notch2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10^6 cells |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2250 and 2300 of human neurogenic locus notch homolog protein 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Neurogenic locus notch homolog protein 2 (NOTCH2) is a highly conserved component of the Notch signaling pathway. It is a type I transmembrane protein with multiple EGF-like repeats in its extracellular domain and several distinct domains in its intracellular region. NOTCH2 acts as a receptor for membrane-bound ligands such as Jagged1, Jagged2, and Delta1, regulating cell-fate determination.
Defects in NOTCH2 are associated with Alagille syndrome type 2 (ALGS2), an autosomal dominant multisystem disorder characterized by hepatic bile duct paucity and cholestasis, along with cardiac, skeletal, and ophthalmologic manifestations. Facial features are distinctive, and renal or vascular involvement may occur.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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