Thermo Fisher Scientific COL4A3 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Target Information

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of three alpha subunits. These subunits are encoded by six different genes (alpha 1 through alpha 6), each capable of forming a triple helix structure with two other subunits to form type IV collagen.
This gene encodes the alpha 3 subunit. In Goodpasture syndrome, autoantibodies bind to collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes eliciting these autoantibodies are largely localized to the non-collagenous C-terminal domain of the protein.
A specific kinase phosphorylates amino acids in this same C-terminal region, and the expression of this kinase is upregulated during pathogenesis.
This gene is also linked to an autosomal recessive form of Alport syndrome, with mutations located within exons encoding this C-terminal region.
Like other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, sharing a common promoter.
[provided by RefSeq, Jun 2010]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.