Thermo Fisher Scientific DAPK1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human DAPK1, validated for WB, IHC, and ICC applications. Recognizes C-terminal region (aa 1360–1389). Supplied as unconjugated liquid at 2 mg/mL. Ideal for research on apoptosis and autophagy pathways.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide between amino acids 1360–1389 from the C-terminal region of human DAPK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2090366 |
Target Information
DAPK1 (Death-associated protein kinase 1) is a large, calmodulin-dependent kinase involved in gamma-interferon–induced programmed cell death and acts as a potential tumor suppressor. It plays roles in multiple cellular signaling pathways that regulate cell survival, apoptosis, and autophagy. DAPK1 mediates both type I apoptotic (caspase-dependent) and type II autophagic (caspase-independent) cell death. It phosphorylates several substrates including PIN1, TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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