Thermo Fisher Scientific PKM2 Polyclonal Antibody
PKM2 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF, ELISA에 사용 가능. Human, Mouse, Rat 반응성. Affinity 정제, PBS/glycerol buffer 보존. 세포질 및 핵 내 PKM2 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 11–221 of PKM (NP_001193728.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.36 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805627 |
Product Specific Information
Immunogen sequence:
SEAGTAFIQT QQLHAAMADT FLEHMCRLDI DSPPITARNT GIICTIGPAS RSVETLKEMI KSGMNVARLN FSHGTHEYHA ETIKNVRTAT ESFASDPILY RPVAVALDTK GPEIRTGLIK GSGTAEVELK KGATLKITLD NAYMEKCDEN ILWLDYKNIC KVVEVGSKIY VDDGLISLQV KQKGADFLVT EVENGGSLGS KKGVNLPGAA V
Positive Samples:
U-251MG, LO2, HeLa, A-549, Mouse kidney, Mouse brain, Mouse lung, Rat brain
Cellular Location:
Cytoplasm, Nucleus
Target Information
PKM2 is a pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP. It interacts with thyroid hormone and may mediate metabolic effects induced by thyroid hormones. PKM2 also binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, suggesting a role in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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