Thermo Fisher Scientific PKM2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 11-221 of PKM (NP_0011937281) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKM2,uniProtId:P14618-1,ncbiNodeId:9606,antigenRange:11-221,antigenLength:531,antigenImageFileName:PA5-89522_PKM2_P14618-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89522_PKM2_P14618-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805627

Product Specific Information

Immunogen sequence: SEAGTAFIQT QQLHAAMADT FLEHMCRLDI DSPPITARNT GIICTIGPAS RSVETLKEMI KSGMNVARLN FSHGTHEYHA ETIKNVRTAT ESFASDPILY RPVAVALDTK GPEIRTGLIK GSGTAEVELK KGATLKITLD NAYMEKCDEN ILWLDYKNIC KVVEVGSKIY VDDGLISLQV KQKGADFLVT EVENGGSLGS KKGVNLPGAA V

Positive Samples: U-251MG, LO2, HeLa, A-549, Mouse kidney, Mouse brain, Mouse lung, Rat brain; Cellular Location: Cytoplasm, Nucleus

Target Information

PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.