Thermo Fisher Scientific mMESSAGE mMACHINE T7 mRNA Kit with CleanCap Reagent AG, 50 reactions

Invitrogen™ mMESSAGE mMACHINE™ T7 mRNA Kit with CleanCap™ Reagent AG

The mMESSAGE mMACHINE T7 mRNA Kit with CleanCap Reagent AG contains all reagents and buffers necessary for in vitro synthesis of large amounts of mRNA with the naturally occurring Cap 1 structure, enabling increased translatability in vivo compared to legacy capping methods such as anti-reverse capping analog (ARCA).
Utilization of the CleanCap Reagent AG ensures superior results relative to older capping technologies and greater protein production.

Each standard 20‑μL reaction yields a minimum of 100 μg of Cap 1-containing mRNA, while the scaled-up protocol yields up to 1 mg of mRNA.
This kit is suitable for full or partially modified-nucleotide substitution to generate modified mRNA.

Features

• Proven CleanCap capping technology
• High mRNA yields (>5 mg/mL)
• Produces mRNA that is over 95% capped
• Optimized for templates 0.9–10 kb
• Yields 100–150 μg per reaction
• Allows use of modified nucleotides (full or partial substitution)
• Includes linearized 0.9 kb GFP control template, yields ≥100 μg mRNA
• Made with TheraPure reagents for transition from RUO to GMP
• Available in 50 and 1000 reaction sizes
  Note: 1000-reaction kit enables ≥100 mg mRNA production.

Description

The kit includes the trinucleotide cap analog CleanCap Reagent AG, which provides the Cap 1 structure for co-transcriptional capping of mRNA without compromising yield.
Cap 1-containing mRNAs are more stable and have superior translational activity compared to Cap 0 mRNAs produced using ARCA or mCap analogs.

A linearized DNA template containing an AG-initiating T7 RNA polymerase promoter is strongly recommended to achieve mRNA yields >5 mg/mL with >90% capping efficiency.

Applications

• Transfection experiments
• Preclinical mRNA therapeutic studies
• Preclinical vaccine development
• Microinjections and in vitro translation
• RNA structure and function analysis

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