Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI3D11), TrueMAB
Ki-67 단백질을 표적하는 마우스 단클론 항체로 세포 증식 마커 분석에 적합. Western blot과 파라핀 조직 면역조직화학(IHC)에 사용 가능. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Miscellaneous PubMed (Misc) | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3D11 |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 1–250 of human MKI67 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly in late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus. Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
This protein is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies help determine the growth fraction in neoplasms and correlate with tumor grade. It is also routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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