Thermo Fisher Scientific CD9 Recombinant Rabbit Monoclonal Antibody (ARC0330)
CD9 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 인간 및 생쥐 반응성, HEK293 세포 발현. 고순도 친화 크로마토그래피 정제, 액상 형태로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0330 |
| Immunogen | Synthetic peptide corresponding to amino acids 129–228 of human CD9 (P21926) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849116 |
Immunogen Sequence
YNKLKTKDEP QRETLKAIHY ALNCCGLAGG VEQFISDICP KKDVLETFTV KSCPDAIKEV FDNKFHIIGA VGIGIAVVMI FGMIFSMILC CAIRRNREMV
Target Information
CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells in the peripheral nervous system. CD9 belongs to the tetraspanin family, which spans the membrane four times. In pre-B cells and platelets, CD9 regulates cell activation and aggregation, possibly through association with integrin CD41/CD61 (GPIIb/GPIIIa). It is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. CD9 plays roles in cell attachment, proliferation, and MHC II molecule association on dendritic cells, crucial for T cell stimulation. It functions in differentiation, adhesion, and signal transduction, and its expression is key in suppressing cancer cell motility and metastasis, acting as a metastasis suppressor in solid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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