Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E10), Biotin, TrueMAB
Biotin으로 결합된 c-Myc 단클론 항체(9E10)로 Western blot, ICC/IF, Flow cytometry에 적합. 인간 단백질에 반응하며, 고순도 Affinity chromatography로 정제됨. 세포 증식 및 종양 연구용으로 최적화된 연구 전용 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E10 |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to C-terminal amino acids 408–432 of human c-Myc |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.1% BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
Product Specific Information
Synthetic peptide EQKLISEEDL conjugated to KLH.
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with regulation of cell proliferation. c-Myc is essential for vasculogenesis and angiogenesis in tumor development.
Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to either amino- or carboxy-termini of target proteins.
Amplification or translocation of the c-Myc gene is observed in various cancers, including lung, breast, colon carcinomas, and Burkitt’s lymphoma. Aberrant c-Myc expression is linked to multiple tumor types across different tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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