Thermo Fisher Scientific ENO1 Monoclonal Antibody (C2)

Applications

Western Blot (WB)

• Tested Dilution: 0.5–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 5–20 µg/mL

Product Specifications

Target Information

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits: alpha, beta, and gamma. Non-neuronal Enolase (ENO1) is an isoform composed of two alpha subunits. The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin.

ENO1 is multifunctional—beyond glycolysis, it contributes to growth control, hypoxia tolerance, and allergic responses. It may act as a receptor and activator of plasminogen on the cell surface of leukocytes and neurons, stimulating immunoglobulin production. ENO1 serves as a diagnostic marker for various tumors and, in heterodimeric alpha/gamma form, as a marker for hypoxic brain injury after cardiac arrest and endometriosis.

Antibodies against alpha-enolase are found in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease linked to systemic tumor growth. ENO1 is also identified as an autoantigen in Hashimoto encephalopathy (HE), a rare autoimmune disease associated with Hashimoto thyroiditis (HT).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.