Thermo Fisher Scientific CD68 Monoclonal Antibody (LAMP4, 824)
CD68 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, Flow Cytometry 등 다양한 응용에 사용 가능. 인간 시료 반응성, Protein A/G 정제, 4°C 보관. 대식세포 및 단핵구 표지 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LAMP4, 824 |
| Immunogen | Recombinant full-length human LAMP4 (CD68) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages, and to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 may play a role in the phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
It is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.
Elevated expression of CD68 has been observed on CD34⁺ cells in various human malignancies, including several AML studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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