Thermo Fisher Scientific XRCC4 Polyclonal Antibody
XRCC4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 IHC(PFA)에서 검증됨. 인간, 마우스, 랫트 시료에 반응하며, Affinity chromatography로 정제됨. DNA 손상 복구 연구 및 V(D)J 재조합 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human XRCC4 (Accession Q13426), corresponding to amino acid residues D234–P284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2900772 |
Product Specific Information
Antibody detects endogenous levels of total XRCC4.
Target Information
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51.
XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination.
Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4, and DNA ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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