Thermo Fisher Scientific Phospho-FAK (Tyr576, Tyr577) Polyclonal Antibody
FAK 단백질의 Tyr576/577 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 액상 형태, 1 mg/mL 농도, PBS 기반 저장용액. 세포 부착 및 신호전달 연구용으로 적합.
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- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- View 1 publication
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:200
- View publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554314 |
Product Specific Information
- Suggested positive control for Western blot: HT29 cells
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase acting as a substrate for Src and a key component of integrin signaling. It plays crucial roles in cell spreading, differentiation, migration, apoptosis, and cell cycle progression (G1 to S phase transition).
FAK localizes to focal adhesions, mediating cell attachment to the extracellular matrix via integrins. Tyrosine phosphorylation of FAK increases upon integrin engagement with fibronectin. Adhesion of NIH3T3 fibroblasts to fibronectin promotes association of Grb2 and c-Src with FAK, activating ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, association of v-Src, Grb2, and Sos with FAK occurs independently of fibronectin adhesion.
The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, with Tyr925 identified as the binding site. Multiple transcript variants exist for FAK, with three full-length isoforms characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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