Thermo Fisher Scientific LFNG Monoclonal Antibody (2D10-3C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

3 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2D10-3C11

Immunogen

LFNG (AAH14851, 1 a.a. approximately 250 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LFNG,uniProtId:Q8NES3-1,ncbiNodeId:9606,antigenRange:1-379,antigenLength:379,antigenImageFileName:H00003955-M01_LFNG_Q8NES3-1_House_mouse.svg,antigenImageFileNamePDP:H00003955-M01_LFNG_Q8NES3-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MTPGRCCLAA DIQVETFIFT DGEDEALARH TGNVVITNCS AAHSRQALSC KMAVEYDRFI ESGRKWFCHV DDDNYVNLRA LLRLLASYPH TRDVYVGKPS LDRPIQAMER VSENKVRPVH FWFATGGAGF CISRGLALKM SPWASGGHFM NTAERIRLPD DCTIGYIVEA LLGVPLIRSG LFHSHLENLQ QVPTSELHEQ VTLSYGMFEN KRNAVHVKGP FSVEADPSRF RSIHCHLYPD TPWCPRTAIF

Target Information

LFNG belongs to evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This protein is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.