Thermo Fisher Scientific RTF1 Polyclonal Antibody
RTF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. IHC, ICC/IF, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human RTF1, amino acids 231–350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65, and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste20, involved in pheromone signaling. The alpha, beta, and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity and leading to autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser19, Ser141, and Thr402, and phosphorylation of Ser141 and Thr402 correlates with gammaPAK activation. Once phosphorylated and with reduced affinity for Rac/Cdc42, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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