Thermo Fisher Scientific ZNF521 Polyclonal Antibody
Human ZNF521 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 및 ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS/glycerol buffer에 보관. 골형성 및 조혈 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ZNF521. Recombinant protein control fragment (Product # RP-93655) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2650171 |
Product Specific Information
Immunogen sequence:
KQDLVKLDIN GLPYGLCAGC VNLSKSASPG INVPPGTNRP GLGQNENLSA IEGKGKVGGL KTRCSSCNVK FESE
Sequence identity:
- Mouse: 88%
- Rat: 85%
Target Information
The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association, and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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