Thermo Fisher Scientific Osteocalcin Monoclonal Antibody (OC4-30)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Goat, Human, Sheep, Pig, Rabbit, Rat

Published species

Dog, Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OC4-30

Immunogen

Full-length Bovine osteocalcin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Osteocalcin,uniProtId:P02820-1,ncbiNodeId:9913,antigenRange:1-100,antigenLength:100,antigenImageFileName:33-5400_Osteocalcin_P02820-1_House_mouse.svg,antigenImageFileNamePDP:33-5400_Osteocalcin_P02820-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

2.0 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533125

Product Specific Information

Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine osteocalcin.

Specificity and Subclass: Epitope gamma-carboxylated residue at position 17 (does not react with decarboxylated osteocalcin.)

Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.

Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

Target Information

Osteocalcin belongs to the osteocalcin/matrix Gla-protein family and constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.