Thermo Scientific™

Pierce™ DSG, No-Weigh™ Format

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface자세히 알아보기

Thermo Scientific Pierce DSG is a water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors. The NHS ester is the simplest and most commonly used reactive group for crosslinking and labeling proteins and peptides. NHS esters react with primary amines on the N-termini of peptides and the  amine of lysine residues, forming a stable, covalent amide bond and releasing the NHS group.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

Features of disuccinimidyl glutarate:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO)
• Membrane-permeable, allowing for intracellular crosslinking
• Can increase crosslinking efficiency compared to DSS in some applications

Product References
Crosslinker Application Guide -- search for recent literature references for this product

사양

화학물질 반응성Amine-Amine

CleavableNo

분자량326.26

페길레이티드No

스페이서 암 길이7.7 Å

세포 투과성Yes

배송 조건Ambient

제품라인Pierce™

라벨링 방법Chemical Labeling

교차결합제 유형Homobifunctional

반응성 부분NHS Ester

스페이서Short (<10 Å)

형태Powder

수량50 mg

용해도DMF, DMSO

형식Standard, Single-use

수용성No

제품 유형Crosslinker

Unit SizeEach