Thermo Fisher Scientific IFN beta Monoclonal Antibody (MMHB-3)

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

MMHB-3

Immunogen

human interferon beta

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion chromatography, Ion-exchange chromatography

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_223445

Product Specific Information

Specificity: Neutralizes human interferon beta. For best results in experiments involving neutralization of the anti-viral effect of human interferon beta we recommend using sheep polyclonal antibody to human interferon beta (Product # 314001).

Activity: Interferon was titrated with the use of the cytopathic effect inhibition assay, where 1 unit/mL of interferon was necessary to produce an endpoint of 50%. One neutralization unit is defined as the amount of serum required to neutralize one unit of human IFN beta to an endpoint of 50%.

Purity is >95%. Endotoxin level <1 EU/ug.

Store at -20°C for short-term use (less than 6 months). For long-term storage, aliquot and store at -70°C or below for retention of full activity, avoiding freeze/thaw cycles.

Packaged by and distributed for PBL Assay Science.

Target Information

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.