Merck Anti-Zyxin antibody, Mouse monoclonal

Anti-Zyxin antibody, Mouse monoclonal

clone ZOL301, purified from hybridoma cell culture

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified immunoglobulin

antibody product type

primary antibodies

클론

ZOL301, monoclonal

form

buffered aqueous solution

분자량

antigen 82-84 kDa

species reactivity

human, mouse, rat

포장

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of HeLa cells.

동형

IgG2a

UniProt 수납 번호

Q15942

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... ZYX(7791)
mouse ... Zyx(22793)
rat ... Zyx(114636)

Anti-Zyxin antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the hybridoma ZOL301 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human Zyxin conjugated to KLH via an N-terminal added cysteine. Zyxin is a relatively low abundance component of focal adhesion plaques, stress fibers, and dynamic cell membrane areas such as the leading edge. It may also be found in the cell nucleus. Zyxin is a member of the LIM domain protein family that also includes LPP (Lipoma preferred partner), Trip6 (thyroid hormone interacting protein 6), and Ajuba. It is a phosphoprotein and comprises two major domains, an extended N-terminal domain that contains proline-rich sequence, and a C-terminal domain that contains a functional nuclear export signal and three double zinc-finger cysteine and histidine-rich LIM domains.