Merck Anti-Neurocan antibody, Mouse monoclonal

Anti-Neurocan antibody, Mouse monoclonal

clone 650.24, purified from hybridoma cell culture

Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified from hybridoma cell culture

antibody product type

primary antibodies

클론

650.24, monoclonal

form

buffered aqueous solution

분자량

antigen 260 kDa (additional band at 160 kDa)

species reactivity

rat

포장

antibody small pack of 25 μL

농도

~1 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.

동형

IgG1

UniProt 수납 번호

P55067

배송 상태

dry ice

저장 온도

−20°C

Gene Information

rat ... Ncan(58982)

Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).