Merck Anti-p62/SQSTM1 antibody produced in rabbit

Anti-p62/SQSTM1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Anti-Ubiquitin-binding p62, Anti-Sequestosome 1

생물학적 소스

rabbit

Quality Level

200

결합

unconjugated

항체 형태

affinity isolated antibody

antibody product type

primary antibodies

클론

polyclonal

form

buffered aqueous solution

분자량

antigen ~62 kDa

species reactivity

human, mouse, rat

포장

antibody small pack of 25 μL

농도

~1 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using lysate of NIH-3T3 cells
indirect immunofluorescence: 1-2 μg/mL using human A549 cells
western blot: 1-2 μg/mL using whole extracts of rat PC12 cells

UniProt 수납 번호

Q13501

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... SQSTM1(8878)
mouse ... Sqstm1(18412)
rat ... Sqstm1(113894)

p62 or SQSTM1 (sequestosome 1) is a multifunctional, multi-domain adaptor protein which resides at the autophagosome membranes. The human p62 gene is mapped to human chromosome 5. The protein has 440 amino acids. This protein is conserved across metazoans, but not plant and fungi. It is composed of Phox1 and Bem1p (PB1) domain, a zinc finger (ZZ) domain, two nuclear localization signals, a tumor necrosis factor receptor-associated factor 6 (TRAF6) binding domain, a nuclear export signal, an LC3-interacting region (LIR), a Keap1-interacting region (KIR) and a ubiquitin-associated (UBA) domain. It is predominantly present in cytoplasm, but also shows expression in nucleus, autophagosomes and lysosomes.
Anti-p62/SQSTM1 is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids of human p62/SQSTM1 (GeneID: 8878), conjugated to KLH. The corresponding sequence is identical in rat and mouse. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.