Merck SPAM1 human

SPAM1 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

PH-20, HYAL3, HYAL5, HYAL1, HYA1, SPAG15

생물학적 소스

human

재조합

expressed in HEK 293 cells

태그

6-His tagged (C-terminus)

assay

≥92% (SDS-PAGE)

form

lyophilized powder

분자량

calculated mol wt 52.1 kDa
observed mol wt 64-66 kDa (DTT-reduced. Protein migrates due to different glycosylation. Leu 36 is the predicted N-terminus.)

포장

pkg of 10 μg
pkg of 50 μg

불순물

<1 EU/μg endotoxin (LAL test)

UniProt 수납 번호

P38567

배송 상태

wet ice

저장 온도

−20°C

Gene Information

human ... SPAM1(6677)

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1→4)-linkages between N – acetyl – β – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.