Merck PNGase F from Elizabethkingia meningoseptica

PNGase F from Elizabethkingia meningoseptica

buffered aqueous solution, recombinant, expressed in E. coli

Peptide N-glycosidase, PNGase F from Flavobacterium meningosepticum, PNGase F from Chryseobacterium meningosepticum, PNGase F from Elizabethkingia meningoseptica, N-Glycosidase F

Quality Level

200

재조합

expressed in E. coli

form

buffered aqueous solution

specific activity

≥1000 U/mg

유통기한

≥1 yr at -20 °C

분자량

~36 kDa

배송 상태

wet ice

저장 온도

−20°C

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.