Merck SIGMAFAST p-Nitrophenyl phosphate Tablets

SIGMA_FAST™_ p-Nitrophenyl phosphate Tablets

tablet, To prepare 20 mL

Quality Level

200

form

tablet

solubility

water: 0.25 tablet/mL, clear, colorless

저장 온도

−20°C

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMA_FAST™_ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.