Merck Peroxidase from horseradish

Peroxidase from horseradish

Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol)

유형

Type VI-A

Quality Level

300

form

essentially salt-free, lyophilized powder

specific activity

≥250 units/mg solid (using pyrogallol)
950-2000 units/mg solid (using ABTS)

분자량

~44 kDa

solubility

0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0)
H2O: soluble

application(s)

diagnostic assay manufacturing

저장 온도

2-8°C

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.