Merck 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

peroxidase substrate

TMB

Quality Level

300

form

liquid

저장 조건

protect from light

배송 상태

wet ice

저장 온도

2-8°C

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.