Merck Ribonuclease A from bovine pancreas

Ribonuclease A from bovine pancreas

Type XII-A, ≥90% (SDS-PAGE), 75-125 Kunitz units/mg protein

RNAsea, RNase A, Pancreatic Ribonuclease, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Quality Level

300

생물학적 소스

bovine pancreas

유형

Type XII-A

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

75-125 Kunitz units/mg protein

분자량

~13,700

불순물

salt, essentially free

외래 활성

protease, essentially free

저장 온도

−20°C

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.