Merck Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas

lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein

Quality Level

300

form

lyophilized powder

specific activity

≥400 Kunitz units/mg protein

분자량

~31 kDa

구성

Protein, ≥85%

solubility

0.15 M NaCl: soluble 5.0 mg/mL, hazy

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

외래 활성

RNase ≤0.02%

배송 상태

wet ice

저장 온도

−20°C

DNase I, or Deoxyribonuclease I, is an endonuclease isolated from bovine pancreas.

• Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
• Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
• DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]