Merck Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum

for general use, Type I, 0.25-1.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Clostridiopeptidase A

Quality Level

200

form

essentially salt-free, lyophilized powder

specific activity

≥125 CDU/mg solid
0.25-1.0 FALGPA units/mg solid

분자량

68,000 to 125,000

최적 pH

6.3-8.8

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 1-2 mg/mL at 37 °C

저장 온도

−20°C

Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family. Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10°C. Crude collagenase mixtures contain two major enzyme types namely, collagenase and clostripain.