Merck Protein A-Sepharose from Staphylococcus aureus

Protein A-Sepharose® from Staphylococcus aureus

lyophilized powder

Protein A–Agarose

Quality Level

200

form

lyophilized powder

포장

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

저장 조건

do not freeze

라벨링 범위

3 mg per mL

technique(s)

affinity chromatography: suitable

색상

white to gray

기질

Sepharose CL-4B

기질 활성

cyanogen bromide

기질 부착

amino

기질 스페이서

1 atom

용량

≥16 mg/mL binding capacity (human IgG)

solubility

water: 1 g/L at 20 °C

적합성

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

저장 온도

2-8°C

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.