Merck Protein G Sepharose, Fast Flow

Protein G Sepharose®, Fast Flow

recombinant, expressed in E. coli, aqueous ethanol suspension

Protein G-Agarose, Fast Flow from Streptococcus sp.

Quality Level

200

재조합

expressed in E. coli

form

aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

라벨링 범위

~2 mg per mL

technique(s)

affinity chromatography: suitable

기질

Sepharose 4B Fast Flow

기질 활성

cyanogen bromide

기질 부착

amino

기질 스페이서

1 atom

저장 온도

2-8°C

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.