Merck HIS-Select Cobalt Affinity Gel

HIS-Select® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Quality Level

200

form

(1:1 suspension in a 30% ethanol solution)

유통기한

1 yr (when stored properly.)

기질

6% Beaded Agarose

용량

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

저장 온도

2-8°C

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.