Merck Glutathione High Capacity Magnetic Agarose Beads

Glutathione High Capacity Magnetic Agarose Beads

(1:1 suspension in a 30% ethanol solution)

Quality Level

200

form

(1:1 suspension in a 30% ethanol solution)

analyte chemical class(es)

proteins (GST)

technique(s)

immunoprecipitation (IP): suitable
protein purification: suitable

기질

4% beaded magnetic agarose

기질 스페이서

12 atoms

용량

≥15 μmol/mL, agarose binding capacity

배송 상태

wet ice

저장 온도

2-8°C

Glutathione Magnetic Agarose Beads consist of a paramagnetic 4% beaded agarose. Glutathione is covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom (10 carbon) spacer.

The Glutathione Magnetic Agarose Beads are useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as native glutathione S-transferase (GST), glutathione peroxidase, and glyoxalase I, or GST-tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non-specific binding of other proteins. Recombinant GST-tagged proteins, bound to the affinity resin are separated with the use of a magnet. The magnetic properties allow for very rapid processing, as well as aid in manipulations, such as repetitive washings and recovery of the desired protein. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest.

The matrix of the magnetic beads is a 4% beaded agarose with an average diameter of 50 μ and a diameter range of 20-75 μ. Paramagnetic iron is impregnated within the beads.