Merck ExtrAvidin-Agarose, high binding

ExtrAvidin®-Agarose, high binding

For purification of biotinylated macromolecules and complexes

form

aqueous suspension
suspension

특징

For purification of biotinylated macromolecules and complexes

농도

50%

기질

4% agarose (beaded)

기질 활성

cyanogen bromide

저장 온도

2-8°C

ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads.
ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii.