Merck HIS-Select Nickel Affinity Gel

HIS-Select® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

Quality Level

200

form

(1:1 suspension in a 20% ethanol solution)

특징

hydrophilic

포장

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

농도

1.5-2.4 mL/mL (suspension in packed gel)

technique(s)

protein purification: suitable

색상

faint blue to very dark blue

기질

6% Beaded Agarose

용량

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

전이 온도

flash point 32 °C (closed cup)

저장 온도

2-8°C

HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.