Merck Biotinylated Molecular Weight Marker

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

form

lyophilized powder

Quality Level

200

사용

vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

분자량

6,500-180,000 Da

포장

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

저장 온도

−20°C

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.