Merck Rat/Mouse Ghrelin (total) ELISA

Rat/Mouse Ghrelin (total) ELISA

This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.

Quality Level

100

species reactivity

rat, mouse

assay range

accuracy: 80-103%
(Serum: Active Ghrelin)
accuracy: 86-102%
(Plasma: Active Ghrelin)
accuracy: 92-103%
(Serum: Des-octanoyl Ghrelin)
accuracy: 95-107%
(Plasma: Des-octanoyl Ghrelin)
inter-assay cv: 1.8-4.5%
intra-assay cv: 0.7-1.3%
(Serum)
inter-assay cv: 2.0-3.2%
intra-assay cv: 1.1-1.7%
(Plasma)

technique(s)

ELISA: suitable

UniProt 수납 번호

Q9EQX0 (mouse)
Q9QYH7 (rat)
Q9UBU3

detection method

colorimetric
fluorometric

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.