Merck Duolink PLA Multicolor Probemaker Kit - Orange

Duolink® PLA Multicolor Probemaker Kit - Orange

Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events

제품 라인

Duolink®

technique(s)

immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable

형광

λex 542 nm; λem 562 nm (orange)
λex 542 nm; λem 562 nm

적합성

suitable for fluorescence

배송 상태

dry ice

저장 온도

−20°C

Specificity
Orange fluorescence detection reagents are often used with Cy®3 filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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