Merck Anti-Laminin antibody, Mouse monoclonal

Anti-Laminin antibody, Mouse monoclonal

clone LAM-89, purified from hybridoma cell culture

Quality Level

200

생물학적 소스

mouse

항체 형태

purified from hybridoma cell culture

antibody product type

primary antibodies

클론

LAM-89, monoclonal

form

buffered aqueous solution

분자량

~850 kDa

species reactivity

feline, porcine, human

향상된 검증

independent
Learn more about Antibody Enhanced Validation

농도

~1.0 mg/mL

technique(s)

ELISA: suitable
electron microscopy: suitable
immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
immunofluorescence: suitable
immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.

동형

IgG1

UniProt 수납 번호

P25391

배송 상태

dry ice

저장 온도

−20°C

Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (850kDa) comprising of an α (400kDa), β (200kDa) and γ (200kDa) chains all held together by disulfide bonds.