Merck Anti-Laminin antibody, Mouse monoclonal
Mouse monoclonal anti-laminin antibody (clone LAM-89), purified from hybridoma culture. Recognizes laminin from human, feline, and porcine origin. Suitable for ELISA, immunoblotting, immunofluorescence, and immunohistochemistry. Stored at −20°C, shippe...
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- SAB4200719-100UL
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- Merck Sigma
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Anti-Laminin Antibody, Mouse Monoclonal (Clone LAM-89)
Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta.
This antibody specifically recognizes laminin from human, feline, and porcine origin. It stains the basal membrane of blood vessels, epithelium, nerve, and muscle fibers, showing no reaction with collagen IV, fibronectin, vitronectin, or chondroitin sulfate types A, B, or C.
Laminins are complex extracellular glycoproteins forming the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850 kDa) composed of α (~400 kDa), β (~200 kDa), and γ (~200 kDa) chains held together by disulfide bonds.
제품 정보
| 항목 | 내용 |
|---|---|
| 클론 | LAM-89 |
| 항체 유형 | Monoclonal, Primary antibody |
| 생물학적 소스 | Mouse |
| 형태 | Buffered aqueous solution |
| 분자량 | ~850 kDa |
| 동형 | IgG1 |
| 농도 | ~1.0 mg/mL |
| Species Reactivity | Human, Feline, Porcine |
| Quality Level | 200 |
| 향상된 검증 | Independent |
| UniProt 번호 | P25391 |
| Technique(s) | ELISA: suitable Electron microscopy: suitable Immunoblotting: 0.03–0.06 μg/mL using laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane Immunofluorescence: suitable Immunohistochemistry: 20–40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections |
| 배송 상태 | Dry ice |
| 저장 온도 | −20°C |
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