Merck Anti-GRP78/BiP (ET-21) antibody produced in rabbit

Anti-GRP78/BiP (ET-21) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

생물학적 소스

rabbit

Quality Level

200

결합

unconjugated

항체 형태

IgG fraction of antiserum

antibody product type

primary antibodies

클론

polyclonal

form

buffered aqueous solution

분자량

antigen 78 kDa

species reactivity

human, Xenopus, hamster, mouse, rat, chicken

향상된 검증

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunocytochemistry: 1:1,000 using methanol/acetone fixed cultured mouse fibroblast NIH3T3 cell line
immunohistochemistry: 1:1,000 using human cerebral cortex and human thyroid gland tissue sections
western blot: 1:3,000 using whole cell extract of human epitheloid carcinoma HeLa cell line

UniProt 수납 번호

P11021

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... HSPA5(3309)
mouse ... Hspa5(14828)
rat ... Hspa5(25617)

Anti-GRP78/BiP (ET-21) antibody produced in rabbit is suitable for use as a primary antibody:

• for immunofluorescence staining of frozen heart tissue to examine whether ER stress signaling activation occurs in cardiomyocytes
• for immunohistochemistry on Paraffin embedded sections of human cerebral cortex and human thyroid gland tissue sections.
• to detect Bip by immunoblotting of protein extract from WT AB strain of zebrafish (Danio rerio)
• at a working dilution of 1:1000 to detect GRP78 in extract of prostate cancer cells after androgen deprivation therapy

It is also suitable for immunoblotting at a working dilution of 1:3000 using human epitheloid carcinoma HeLa whole cell extract and for immunocytochemistry at a working dilution of 1:1000 using mouse fibroblasts NIH3T3 cells.