Merck Pwo Master

Pwo Master

sufficient for ≤100 reactions, pkg of 2.5 mL (10 x 250 μl)

High fidelity PCR | PCR | polymerase

Quality Level

100

사용

sufficient for ≤100 reactions

포장

pkg of 2.5 mL (10 x 250 μl)

manufacturer/tradename

Roche

파라미터

72 °C optimum reaction temp.

배송 상태

wet ice

저장 온도

2-8°C

Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3′–5′ exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.