Merck hGH ELISA

hGH ELISA

sufficient for 192 tests, >98% (SDS-PAGE), kit of 1, suitable for ELISA

ELISA

Quality Level

100

assay

>98% (SDS-PAGE)

사용

sufficient for 192 tests

포장

kit of 1

manufacturer/tradename

Roche

technique(s)

ELISA: suitable

배송 상태

wet ice

저장 온도

2-8°C

The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g., RNA isolation). Additionally, hGH is a mammalian gene that may contribute to the apparent high stability of the hGH mRNA in most mammalian cells.
Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. Usually, hGH is quantified by an immunoradiometric assay (IRMA), resulting in a linear range of approximately 0.1 to 50ng hGH/ml.