cOmplete™ Lysis-M EDTA-free
manufacturer/tradename
Roche
배송 상태
ambient
The cOmplete® Lysis-M EDTA-free mammalian cell protein extraction reagent contains a mild detergent (in 25mM bicine buffer, pH 7.6). cOmplete Lysis-M EDTA-free is intended for the efficient and gentle extraction of proteins from both the cytoplasm and the nucleus of cultured mammalian cells. Efficient lysis of mammalian cells occurs in only 5 minutes at room temperature, eliminating the need for scraping, sonication, or freeze-thaw cycles.
The ready-to-use cOmplete Mini EDTA-free Protease Inhibitor Cocktail Tablets, included in the kit, work optimally with the kit ′s Lysis-M reagent. cOmplete Lysis-M EDTA-free provides a very convenient and efficient way to extract proteins and simultaneously inhibit protease activity during the initial extraction steps. cOmplete Mini EDTA-free Protease Inhibitor Cocktail Tablets contains both irreversible and reversible protease inhibitors. cOmplete Mini EDTA-free tablets efficiently inhibit a wide range of serine and cysteine proteases, but not metalloproteases.
The extracted proteins can be further purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed in downstream applications.
