Merck Pefabloc SC PLUS

Pefabloc® SC PLUS

crystalline, suitable for blocking, suitable for protein purification, 0.1-1.0 mg/mL

pefabloc, serine protease inhibitor

Quality Level

100

form

crystalline

분자량

239.5

포장

pkg of 5 mL (100 mg)

manufacturer/tradename

Roche

농도

0.1-1.0 mg/mL

technique(s)

blocking: suitable
protein purification: suitable

최적 pH

neutral

mp

175-185 °C

solubility

soluble (Solubility and stability of the protease inhibitor remains unchanged.)

배송 상태

wet ice

저장 온도

2-8°C

Pefabloc SC is a specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity is comparable to PMSF or DFP, but Pefabloc SC is nontoxic to cells. The Pefabloc SC PLUS set combines the serine protease inhibitor Pefabloc SC with a uniquely formulated Pefabloc SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc SC.

Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.