Merck 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit I

sufficient for ≤100 tests, kit of 1 (5 components), suitable for immunofluorescence

5-BrdU, 5-Bromo-2-deoxyuridine

Quality Level

100

사용

sufficient for ≤100 tests

포장

kit of 1 (5 components)

manufacturer/tradename

Roche

technique(s)

immunofluorescence: suitable

배송 상태

dry ice

저장 온도

−20°C

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.
Normally, binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid, base, or heat. These procedures result in destruction of cell integrity, including cell morphology and surface and cytoplasmatic markers.
The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.