Merck β-Gal Staining Set

β-Gal Staining Set

solution, sufficient for 100 tests (in 3.5 cm dishes)

immunofluorescence, staining kit

form

solution

Quality Level

100

사용

sufficient for 100 tests (in 3.5 cm dishes)

manufacturer/tradename

Roche

배송 상태

wet ice

저장 온도

2-8°C

The β-Gal Staining Set detects bacterial β-Gal activity for direct visualization in transfected cells. Forms a blue precipitate and simplifies the histochemical staining of cells and tissue sections for light microscopy. Upon transfecting cells with a β-Gal construct (encoded by the lacZ gene), cells are fixed, washed in PBS (phosphate buffered saline) and stained with freshly prepared staining solution. After staining, cells are washed again in PBS and evaluated by microscopy. When long-term storage is desired, PBS is replaced by mounting media (e.g., glycerol).
For immunohistochemical staining of cells or tissue sections, incubate the specimen with an antibody conjugated to bacterial β-galactosidase, according to standard protocols. Subsequently, the specimen is processed for detection of β-Gal as described for transfected cells.