Merck Transcriptor Universal cDNA Master

Transcriptor Universal cDNA Master

sufficient for 100 reactions, suitable for cDNA synthesis, suitable for RT-PCR, suitable for RT-qPCR

RT-PCR | cDNA synthesis

Quality Level

100

사용

sufficient for 100 reactions

특징

dNTPs included: no
hotstart: no

manufacturer/tradename

Roche

포장

kit of for 100 reactions

technique(s)

RT-PCR: suitable
RT-qPCR: suitable
cDNA synthesis: suitable

input

purified RNA

detection method

probe-based

Ready-to-use reaction mix for two-step RT-qPCR on all real-time PCR systems.

The Transcriptor Universal cDNA Master is the convenient solution for time-saving cDNA synthesis for two-step real-time RT-PCR. All reagents required, including random hexamer primer, nucleotides, buffers and enzymes are supplied in just two vials minimizing pipetting. Maximum convenience is achieved by combining the Transcriptor Universal cDNA Master mix with the RealTime ready Cell Lysis Kit, further accelerating real-time PCR by directly using cell lysates. The unique enzyme included in this kit has a broad temperature range and is suitable for high temperature reverse transcription. The Transcriptor Universal cDNA Master produces very high specificity and sensitivity, even with difficult complex RNA templates. Excellent performance and linear response in real-time RT-PCR is obtained for both low and high copy genes. The Transcriptor Universal cDNA Master is compatible with LightCycler® instruments and all other real-time PCR instruments requiring normalization with a reference dye.