Merck DNA, lambda

DNA, lambda

from bacteriophage lambda cl857 Sam 7

lambda dna

Quality Level

100

form

solution

분자량

32,300 kDa

포장

pkg of 1 mL

manufacturer/tradename

Roche

저장 조건

avoid repeated freeze/thaw cycles

배송 상태

dry ice

저장 온도

−20°C (−15°C to −25°C)

The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.